ABSTRACT
Objective To study the relationship of the levels of serum ET-1,MCP-1 and MMP-9 in patients with different type coronary heart disease (CHD).Methods 80 CHD patients were divided into 3 groups,including 22 cases of acute myocardial infarction (AMI),33 cases of unstable angina (UAP),25 cases of stable angina (SAP) and control group consisted of 30 healthy people.The levels of ET-1,MCP-1 and MMP-9 were measured by enzymelinked immunosorbent assay (ELISA).The levels of serum ET-1,MCP-1 and MMP-9 in different groups were compared.Results The serum levels of ET-1,MCP-1 and MMP-9 in AMI,UAP and SAP groups were significantly higher than those in the control group (t =5.12,5.35,5.15,4.22,4.65,4.54,3.12,3.65,3.54,all P ＜ 0.05).Compared with SAP group,the levels of ET-1,MCP-1 and MMP-9 were increased in AMI and UAP groups (t =4.32,4.15,4.52,3.54,3.23,3.51,all P ＜ 0.05).Compared with UAP group,the levels of ET-1,MCP-1 and MMP-9 were increased in AMI groups (t =3.78,3.96,3.56,all P ＜ 0.05).Conclusion Serum ET-1,MCP-1 and MMP-9 may be the markers of coronary atherosclerosis and involved in the process of CHD.The levels of serum ET-1,MCP-1 and MMP-9 are correlated with the severity of CHD.
ABSTRACT
Objective To explore changes in glycogen synthase kinase-3 (GSK-3) levels in the lymphocytes of patients with mild cognition impairment (MCI) and Alzheimer's disease (AD). Methods GSK-3 activity was measured by 32P liquid scintillography in a control group, an MCI group and an AD group. The expressions of GSK-3βat Ser9 and total GSK-3β were determined by western blotting. Results GSK-3 levels were significantly higher in the AD and MCI groups than in the control group. The phosphorylation of GSK-3βat Ser9 was significantly lower in the AD and MCI groups compared with the control group, but there was no difference in the phosphorylation of total GSK-3β among the three groups. Conclusions Higher GSK-3 activity in the lymphocytes might have value in the diagnosis of MCI and early AD.